[HTML][HTML] RAD marker microarrays enable rapid mapping of zebrafish mutations
Genome Biology, 2007•Springer
We constructed a restriction site associated DNA (RAD) marker microarray to facilitate rapid
genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant
approach, we localized previously unmapped mutations to genomic regions just a few
centiMorgans in length. Furthermore, we developed an approach to assay individual RAD
markers in pooled populations and refined one region. The RAD approach is highly effective
for genetic mapping in zebrafish and is an attractive option for mapping in other organisms.
genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant
approach, we localized previously unmapped mutations to genomic regions just a few
centiMorgans in length. Furthermore, we developed an approach to assay individual RAD
markers in pooled populations and refined one region. The RAD approach is highly effective
for genetic mapping in zebrafish and is an attractive option for mapping in other organisms.
Abstract
We constructed a restriction site associated DNA (RAD) marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and is an attractive option for mapping in other organisms.
Springer
以上显示的是最相近的搜索结果。 查看全部搜索结果